Skip to main content
. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Pain. 2012 Aug 31;153(11):2283–2291. doi: 10.1016/j.pain.2012.07.037

Fig. 5.

Fig. 5

Role of estrogen in CB1R expression in trigeminal ganglia (TG) from female rats. (A) CB1R mRNA levels were assessed from TG of ovariectomized (OVX) rats treated with cytokines. (B) Estradiol benzoate (ES) alone or ES combined with cytokines were applied to TG cultures from intact female rats and CB1R mRNA levels assessed. (C) An estrogen receptor antagonist, ICI-182,780 (ICI) alone or ICI combined with cytokines were applied to TG cultures from intact female rats and CB1R mRNA assessed. All data are normalized to untreated samples and presented as mean percent change in CB1R mRNA transcripts. Data are shown as mean ± SE and each group consisted of 5–6 replicates. *denotes significant differences compared to PBS group at P < 0.05. PBS, phosphate-buffered saline; IL, interleukin; TNF, tumor necrosis factor.