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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Pain. 2012 Aug 31;153(11):2283–2291. doi: 10.1016/j.pain.2012.07.037

Fig. 6.

Fig. 6

Role of testosterone in CB1R mRNA expression in trigeminal ganglia (TG) from male rats. (A) Real-time polymerase chain reaction data following individual or combination of cytokines applications were compared to those of PBS treatment in orchidectomized (GDX) rats. (B) Testosterone (TS) alone or TS combined with cytokines were applied to TG cultures from GDX rats and CB1R mRNA levels assessed. All data are normalized to untreated samples and presented as mean percent change in CB1R mRNA transcripts. Data are shown as mean ± SE and each group consisted of 5–6 replicates. *denotes significant differences compared to PBS group at P < 0.05. (C) Effects of arachidonylcyclopropylamide (ACPA) on masseter hypersensitivity 3 days after complete Freund’s adjuvant treatment in GDX rats. Bar graphs show mean % change in EF50 values in vehicle (phosphate-buffered saline [PBS])- and ACPA-treated rats. Data are shown as mean ± SE and each group consisted of 6 animals.