Table 1.
Connexin PCR primers
| Connexin | Upstream/Downstream Sequences | Annealing Temp. (°C) |
Amplicon Length (bp) |
|---|---|---|---|
| Cx26 | 5'-CGGAAGTTCATGAAGGGAGAGAT-3' | 60 | 365 |
| 5'-GGTCTTTTGGACTTTCCTGAGCA-3' | |||
| Cx29 | 5'-GCTCATGGGATTCCGTCTC-3' | 55 | 460 |
| 5'-GGTTGTGCTGCCAATACAAGG-3' | |||
| Cx30 | 5'-AATGTGGCCGAGTTGTGTTA-3' | 53 | 399 |
| 5'-CCAAGGCCCAGTTGTCAC-3' | |||
| Cx30.2 | 5'-TCATGCTGATCTTCCGCATC-3' | 55 | 218 |
| 5'-GGCCTGGTGCATGGAGTAGAT-3' | |||
| Cx30.3 | 5'-TCAAACATGGGCCCAATG-3' | 60 | 182 |
| 5'-GGGAGTCACAGAGCAAGC-3' | |||
| Cx31 | 5'-AGAAGCACGGGGAGCAAT-3' | 60 | 182 |
| 5'-TACTATGCTGGCGCACTG -3' | |||
| Cx31.1 | 5'-ATATACCCTCCCTTCTATGGT-3' | 55 | 357 |
| 5'-TCACAGAATGGTTTTCTTCA-3' | |||
| Cx32 | 5'-CTGCTCTACCCCGGCTATGC-3' | 60 | 386 |
| 5'-CAGGCTGAGCATCGGTCGCTCTT-3' | |||
| Cx33 | 5'-GAGGCAGATTGCTGCTAACC-3' | 56 | 323 |
| 5'-AGGGCATGGTAGCTCATCAC-3' | |||
| Cx36 | 5'-GGGGTGCTGCAGAACACAGAGA-3' | 60 | 344 |
| 5'-ACCACACAAATGCCGCTCACA-3' | |||
| Cx37 | 5'-GGCTGGACCATGGAGCCGGT-3' | 60 | 422 |
| 5'-TTTCGGCCACCCTGGGGAGC-3' | |||
| Cx39 | 5'-TTGCTCGCATGTACCCTG-3' | 55 | 265 |
| 5'-CACCTGACCTTGGCTAAGATA-3' | |||
| Cx40 | 5'-CTGTCCCCACCCAGTCAACT-3' | 57 | 460 |
| 5'-CGGTTTGTCACTATGGTAGC-3' | |||
| Cx43 | 5'-TACCACGCCACCACCGGCCCA-3' | 60 | 294 |
| 5'-GGCATTTTGGCTGTCGTCAGGGAA-3' | |||
| Cx45 | 5'-TTCCAAGTCCACCCATTTTAT-3' | 55 | 444 |
| 5'-ATCGTTCCTGAGCCATTCTGA-3' | |||
| Cx46 | 5'-GGAAAGGCCACAGGGTTTCCTGG-3' | 60 | 332 |
| 5'-GGGTCCAGGAGGACCAACGG-3' | |||
| Cx47 | 5'-ATGGGATTGCTTCGTGTCGC-3' | 60 | 256 |
| 5'-ACGCACCACCAGGCTGTAGTCTG-3' | |||
| Cx50 | 5'-CTTTGTATCCCGGCCTACTGA-3' | 60 | 466 |
| 5'-CTTCTCTCCCACTTCCGGTTCCA-3' | |||
| Cx57 | 5'-CCACATGACCGCAGTG-3' | 60 | 392 |
| 5'-GGTGATGGGCTGTTTTCT-3' |