Figure 2.

Representations of AdV and ϕ29 polymerases showing the locations of conserved sequence motifs and structural domains. The domain organization shown for ϕ29 Pol was described previously by Kamtekar et al. (2004) and is based on structural data. The domain organization depicted for AdV Pol is inferred from that of ϕ29 Pol and is based on the relative location of motifs shared among (many of) the DNA polymerases. Indicated for both polymerases are the most highly conserved of these sequence motifs within the exonuclease domain (“Exo” motifs I, II, and III) and the polymerase region (“Pol” motifs I, II, and III, also known as, respectively, motifs C, A, and B) (Wong et al. 1988; Bernad et al. 1989; Delarue et al. 1990). All representations are to scale.