FIG. 1.

TIP110 and C-MYC share similar expression patterns. Semiquantitative RT-polymerase chain reaction (PCR) of: (a) Freshly isolated CD34⁺ cells (L1), CD34⁺ cells cultured in the Iscove's modified Dulbecco's medium (IMDM) with fetal bovine serum, stem cell factor, thrombopoietin, and Fms-related tyrosine kinase 3 ligand (FL) for 24 h (L2), or cells continued in culture for 8 days (L3); (b) K562 cells cultured in the RPMI medium (L1) or included with 20 nM phorbol 12-myristate 13-acetate for 5 days (L2). (c) ESC line (H9) cultured to yield undifferentiated human embryonic stem cells (hESCs) (L1) or differentiated hESC offspring under feeder-free conditions by withdrawing basic fibroblast growth factor in a medium for 3 days (L2). β-actin was used as a loading control. Data shown are from 1 of at least 3 reproducible experiments.