Fig. 1.
re-S.c. TIM. (a) Histogram of relative entropy values for all 240 aligned positions in the TIM family. The mean RE is 1.42. (b) The six most conserved positions in S.c. TIM that are not consensus amino acids are shown in green sticks. The active-site residues are shown in orange on the 1YPI crystal structure. (c) Ellipticity at 222 nm is followed with increasing temperature. The wild type melts at 59.1 °C, but re-S.c. TIM melts at 57.0 °C.