Fig. 3.

Thermal stabilities of consensus TIM variants. We monitored the loss of secondary structure with increasing temperature at 222 nm for helices (a) and 215 nm for sheets (b). (c) The optical density at 600 nm from aggregation reports similar two-state unfolding profiles as the CD thermal melts. (d) High-throughput thermal scanning was used to assay the melting temperatures based on hydrophobic dye binding. Note that the same colors are used for the same variants in (a)–(d).