FIG. 2.

Induction of HSP 70 normalized with alpha-tubulin in normal human skin fibroblasts treated for 6 hr with 750 nM celastrol (positive control) and 250 μg /ml Sanchi extract. Monoclonal mouse anti-human Hsp70 antibody (1/1500 dilution; Gentaur) and sheep ECL anti-mouse IgG HRP (1/200,000 dilution; GE Healthcare-Amersham) were used for western blotting of Hsp70 (A). Western blot imaging and quantification was done by ImageJ software (B). Statistical analysis was performed with STATGRAPHICS PLUS CENTURION package UNIWIN 6.0, with results of 3 independent experiments. Levels of Hsp70 were increased significantly (p<0.05), by 25% and 24% in celastrol- and Sanchi-treated cells, respectively.