Table 3.
Cox Proportional Hazard Analysis Including MSP and IHC Combinations with Bootstrap Validation
| Overall Survival | Overall Survival (Bootstrap) | Progression Free Survival | Progression Free Survival (Bootstrap) | ||
|---|---|---|---|---|---|
| Factor | Estimate HR (95% CI), P-value | Estimate HR (95% CI), P-value | Estimate HR (95% CI), P-value | Estimate HR (95% CI), P-value | |
| MGMT MSP and IHC | 1. MSP = M, IHC < 30 (n = 100) | 1.92 (1.18, 3.14), P = .0087 | 1.92 (1.18, 3.14), P = .0087 | 1.92 (1.18, 3.14), P = .0087 | 1.92 (1.18, 3.14), P = .0087 |
| 2. MSP = M, IHC≥30 (n = 29) | 1.92 (1.18, 3.14), P = .0087 | 1.91 (1.16, 3.16), P = .0114 | 1.78 (1.10, 2.88), P = .0186 | 1.82 (1.13, 2.93), P = .0132 | |
| 3. MSP = U, IHC < 30 (n = 79) | 2.20 (1.54, 3.12), P < .0001 | 2.14 (1.47, 3.12), P < .0001 | 1.98 (1.41, 2.79), P < .0001 | 2.02 (1.41, 2.90), P = .0001 | |
| 4. MSP = U, IHC≥30 (n = 136) | 2.62 (1.91, 3.60), P < .0001 | 2.57 (1.84, 3.60), P < .0001 | 2.15 (1.59, 2.92), P < .0001 | 2.23 (1.60, 3.10), P < .0001 | |
| Gender | Male vs. Female | 1.48 (1.15, 1.91), P = .0021 | 1.47 (1.10, 1.97), P = .0091 | 1.30 (1.02, 1.67), P = .0371 | 1.28 (0.98, 1.68), P = .0691 |
| Age | 1.02 (1.01, 1.04), P < .0001 | 1.02 (1.01, 1.04), P < .0001 | 1.02 (1.01, 1.03), P = .0039 | 1.02 (1.01, 1.03), P = .0046 | |
| KPS | 100 | 1.48 (1.15, 1.91), P = .0021 | 1.48 (1.15, 1.91), P = .0021 | 1.48 (1.15, 1.91), P = .0021 | 1.48 (1.15, 1.91), P = .0021 |
| 90 | 1.21 (0.83, 1.75), P = 0.3246 | 1.24 (0.87, 1.78), P = .2310 | 1.05 (0.73, 1.52), P = .7908 | 1.04 (0.74, 1.46), P = .8306 | |
| 80 | 1.24 (0.82, 1.88), P = .2989 | 1.27 (0.85, 1.88), P = .2384 | 0.91 (0.60, 1.38), P = .6595 | 0.90 (0.63, 1.28), P = .5544 | |
| 70 or lower | 1.91 (1.19, 3.05), P = .0070 | 2.03 (1.08, 3.83), P = .0279 | 1.68 (1.05, 2.68), P = .0309 | 1.72 (1.04, 2.84), P = .0336 | |
| Resection | GTR vs. STR and Bx | 0.68 (0.53, 0.88), P = .0028 | 0.66 (0.50, 0.87), P = .0028 | 0.65 (0.51, 0.84), P = .0008 | 0.64 (0.49, 0.83), P = .0006 |
| Bevacizumab | Yes vs. No | 0.78 (0.60, 0.99), P = .0444 | 0.75 (0.57, 0.99), P = .0418 | 1.07 (0.84, 1.37), P = .5778 | 1.08 (0.84, 1.38), P = .5464 |
| IDH1 | WT vs. MUT | 0.52 (0.26, 1.01), P = .0528 | 0.50 (0.25, 0.97), P = .0416 | 0.59 (0.32, 1.08), P = .0867 | 0.59 (0.31, 1.11), P = .1015 |
Abbreviations: Bx, biopsy; GTR, gross-total resection; IHC, immunohistochemistry; KPS, Karnofsky performance status; MGMT, O6-methylguanine DNA methyltransferase; MSP, methylation specific PCR; M, methylated; MUT, mutant; STR, sub-total resection; U, unmethylated; WT, wildtype.