Figure 3.

IndOH-LNC decrease the pAKT protein immunocontent in glioma cells. Western blotting for pAKT, AKT, and β-actin was performed at the indicated time in 25 μM IndOH-LNC-treated (A) C6 and (B) U138-MG glioma cells.

Notes: Unloaded LNC were considered the vehicle control of IndOH-LNC. The histogram presents the quantitative Western blot analysis of the AKT phosphorylation state. The densitometric values obtained for phospho- and total-AKT after treatments were normalized to their respective controls (control bar) (100%). Data are expressed as a ratio of the normalized percentages of pAKT and AKT. Bars represent the mean ± standard deviation for six independent experiments. significant differences from control and LNC groups: **P< 0.01 and ***P< 0.001, as assessed by one-way analysis of variance followed by the Bonferroni post hoc test.

Abbreviations: IndOH-LNC, indomethacin-loaded lipid-core nanocapsules; LNC, lipid-core nanocapsules.