Table 3. Number of minutes required to align sequences and calculate distances between sequences using various algorithms.

Alignment algorithm	V13	V35
NAST-greengenes	17.4	10.3
NAST-SILVA	19.2	15.7
Infernal-RDP	27.6	24.4
Needleman–Wunsch	1321.1	1081.5
Gotoh	1516.1	1221.2

Abbreviations: Infernal, inference of RNA alignment; NAST, Nearest Alignment Space Termination; RDP, Ribosomal.Database Project. All times are based on using a single processor although all algorithms can be parallelized. The alignment algorithms are sorted according to the number of minutes required.