Table 1. Isolate grouping and the relative frequency of antagonism, sensitivity and resistant phenotypes.
Groupa | Type of bacteria in the group | No. of isolates in each group | Isolates antagonistic to at least one other isolate | HAb | Sensitive to atleast one other isolate | HSc | fr A | fr HA | fr S | fr HS |
---|---|---|---|---|---|---|---|---|---|---|
A | B. pumilus | 14 | 10 | 7 | 8 | 2 | 0.71 | 0.5 | 0.57 | 0.14 |
B | B. subtilis | 4 | 4 | 4 | 2 | 0 | 1 | 1 | 0.5 | 0 |
C | B. alcalophilus | 7 | 1 | 0 | 7 | 5 | 0.14 | 0 | 1 | 0.71 |
D | B. aquimaris | 7 | 5 | 0 | 5 | 4 | 0.71 | 0 | 0.71 | 0.57 |
E | B. NRRLB14911 | 9 | 6 | 2 | 8 | 7 | 0.66 | 0.22 | 0.88 | 0.77 |
F | B. cereus | 12 | 6 | 0 | 6 | 0 | 0.5 | 0 | 0.5 | 0 |
G | B. horikoshii 633 | 13 | 2 | 1 | 11 | 7 | 0.15 | 0.08 | 0.84 | 0.54 |
H | Diverse Bacillus spp. | 6 | 4 | 1 | 5 | 2 | 0.66 | 0.16 | 0.83 | 0.33 |
I | Staphylococcus arlettae, S. succinus | 2 | 1 | 1 | 0 | 0 | 0.5 | 0.5 | 0 | 0 |
J | Diverse Actinobacteria | 4 | 0 | 0 | 4 | 2 | 0 | 0 | 1 | 0.5 |
Total | 78 | 39 | 16 | 56 | 29 | 0.5 | 0.2 | 0.72 | 0.37 |
Abbreviations: A, antagonistic; HA, highly antagonistic; HS, highly sensitive; S, sensitive.
Most Bacillus groups are related and have at least 97% identity. This classification was based on a 16S rRNA gene alignment, and Mothur was used to generate the phylotype clusters (see Materials and methods). Note that the similarity is not based on the full 16S rRNA gene sequence. Group H includes all divergent Bacillus isolates that have no known strain sequence and that is close enough for comparison (isolates 148, 449a1, 445 and 41b), as well as isolates related to B. indicus (450), B. sp. JAMB-204 (113a), B. megaterium (447) and B. thuringiensis (155a).
HA: isolates that can antagonize 10 or more other isolates were considered highly antagonistic.
HS: isolates that are sensitive to attack from 10 or more other isolates were considered highly sensitive.