Table 1. Constituents of the artificial medium for SAR11 (AMS1)a.
| Compound | Final concentration |
|---|---|
| Base salts | |
| NaCl | 481 mℳ |
| MgCl2·6H2O | 27 mℳ |
| CaCl2·2H2O | 10 mℳ |
| KCl | 9 mℳ |
| NaHCO3 | 6 mℳ |
| MgSO4·7H2O | 2.8 mℳ |
| Macronutrients | |
| (NH4)2SO4 | 400 μℳ |
| NaH2PO4 (pH 7.5) | 50 μℳ |
| Trace metals | |
| FeCl3·6H2O | 117 nℳ |
| MnCl2·4H2O | 9 nℳ |
| ZnSO4·7H2O | 800 pM |
| CoCl2·6H2O | 500 pM |
| Na2MoO4·2H2O | 300 pM |
| Na2SeO3 | 1 nℳ |
| NiCl2·6H2O | 1 nℳ |
| Vitamins | |
| B1 | 6 μℳ |
| B3 | 800 nℳ |
| B5 | 425 nℳ |
| B6 | 500 nℳ |
| B7 | 4 nℳ |
| B9 | 4 nℳ |
| B12 | 700 pM |
| Myo-inositol | 6 μℳ |
| 4-Aminobenzoic acid | 60 nℳ |
a
The AMS1 medium does not include nutrients to meet the requirements for reduced sulfur (see Tripp et al., 2008), glycine (see text) or pyruvate (see text).