Table 1. Primers used to screen for presence of genes in the flexible genome of V. cyclotrophicus (sub)populations II.A and II.B.

Name	Direction	Annealing temperature (°C)	Sequence (5′-3′)
Flex1	F	54.6	GTCAAAACACGCTCTCCA
	R	54.6	TCGATCTCGCACAAAACT
Flex2	F	48.2	TTTTGTTATTTGGAGTGTG
	R	48.2	CTTTTTCGAGCAATATATC
Flex3	F	51.2	GCATGATCACCTCCACGA
	R	51.2	ACCTACCCCACAAATACT
Flex4	F	52.2	ACACCTTACATCGGATCA
	R	52.2	GTACAACCACACTTTTCCT
Flex5	F	55.0	CGCTTCGCACTCTTTAACA
	R	55.0	CCACGCAGCATATCCAAT