Table 3. PCR screen to test for distribution of genes in the flexible genome of V. cyclotrophicus (sub)populations II.A and II.B obtained in 2009 demonstrating clear genotypic differentiation.
Population | Isolate |
Genea |
|||||
---|---|---|---|---|---|---|---|
hsp60b | flex1 | flex2 | flex3 | flex4 | flex5 | ||
II.A | 1F_A1634 | + | − | + | − | − | − |
1F_A33 | + | + | + | + | − | − | |
1F_A1560 | + | + | + | + | + | + | |
1F_A1433 | + | + | + | + | + | + | |
1F_A1553 | + | − | − | − | − | + | |
5F_A1948 | + | + | + | + | + | + | |
5F_A1931 | + | + | + | + | + | + | |
5F_A1944 | + | + | + | + | + | + | |
5F_A56 | + | − | − | − | − | − | |
5F_A93 | + | + | + | + | + | + | |
FF_A1147 | + | + | + | + | + | + | |
FF_A1115 | + | + | + | + | + | + | |
FF_A1149 | + | − | + | − | − | − | |
FF_A1066 | + | + | + | + | + | + | |
FF_A1245 | + | + | + | + | + | + | |
ZF_A738 | + | + | + | + | + | + | |
ZF_A453 | + | + | + | + | + | + | |
ZF_A773 | + | + | + | + | + | + | |
ZF_A812 | + | + | + | + | + | + | |
ZF_A588 | + | + | + | + | + | + | |
II.B | FF_A1219 | + | − | − | − | − | − |
FF_A1319 | + | − | − | − | − | +/− | |
1F_A1380 | + | − | + | − | − | − | |
1F_A1444 | + | − | − | − | − | +/− | |
5F_A1731 | + | − | − | − | − | − |
Primers are shown in Table 1; +, − and +/− indicate strongly positive, negative and weak bands in PCR screens.
Amplification of the hsp60 gene served as positive control.