Table 3. PCR screen to test for distribution of genes in the flexible genome of V. cyclotrophicus (sub)populations II.A and II.B obtained in 2009 demonstrating clear genotypic differentiation.

Population	Isolate	Genea
		hsp60b	flex1	flex2	flex3	flex4	flex5
II.A	1F_A1634	+	−	+	−	−	−
	1F_A33	+	+	+	+	−	−
	1F_A1560	+	+	+	+	+	+
	1F_A1433	+	+	+	+	+	+
	1F_A1553	+	−	−	−	−	+
	5F_A1948	+	+	+	+	+	+
	5F_A1931	+	+	+	+	+	+
	5F_A1944	+	+	+	+	+	+
	5F_A56	+	−	−	−	−	−
	5F_A93	+	+	+	+	+	+
	FF_A1147	+	+	+	+	+	+
	FF_A1115	+	+	+	+	+	+
	FF_A1149	+	−	+	−	−	−
	FF_A1066	+	+	+	+	+	+
	FF_A1245	+	+	+	+	+	+
	ZF_A738	+	+	+	+	+	+
	ZF_A453	+	+	+	+	+	+
	ZF_A773	+	+	+	+	+	+
	ZF_A812	+	+	+	+	+	+
	ZF_A588	+	+	+	+	+	+
II.B	FF_A1219	+	−	−	−	−	−
	FF_A1319	+	−	−	−	−	+/−
	1F_A1380	+	−	+	−	−	−
	1F_A1444	+	−	−	−	−	+/−
	5F_A1731	+	−	−	−	−	−

^aPrimers are shown in Table 1; +, − and +/− indicate strongly positive, negative and weak bands in PCR screens.

^bAmplification of the hsp60 gene served as positive control.