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. Author manuscript; available in PMC: 2014 Jan 14.
Published in final edited form as: Cancer Cell. 2012 Dec 27;23(1):107–120. doi: 10.1016/j.ccr.2012.11.013

Figure 2. Deletion of SphK2 increases S1P and the severity of colitis.

Figure 2

(A–D) Acute colitis was induced in Sphk2−/− mice and WT littermates with 5% DSS in drinking water for 5 days followed by recovery for 5 days on normal drinking water. Changes in body weight (A), colon shortening (B), mucosal histology examined by H&E staining (C, scale bar, 100 μm), and colitis severity scores were determined (D) in a double-blind manner. In (A), (B), and (D), data are means ± SEM (n=6). *p<0.05, **p<0.001 compared to WT treated with DSS.

(E,F) S1P in serum (E) and colon (F) was measured by LC-ESI-MS/MS.

(G,H) Expression of SphK1 and SphK2 in colons was determined by QPCR (G) and western blotting (H).

In (E–G), data are means ± SD (n=3). *p<0.05, **p<0.005.

See also Figure S1.