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. Author manuscript; available in PMC: 2014 Jan 14.
Published in final edited form as: Cancer Cell. 2012 Dec 27;23(1):107–120. doi: 10.1016/j.ccr.2012.11.013

Figure 3. Severity of colitis in Sphk2−/− mice correlates with upregulation of SphK1, S1PR1, and IL-6, and activation of NF-κB and STAT3.

Figure 3

(A) Colonic lysates or nuclear extracts from Sphk2−/− mice and WT littermates were analyzed by immunoblotting with the indicated antibodies.

(B) WT and Sphk2−/− MEFs were pretreated without or with SAHA, TSA, or SP600125, stimulated without or with PMA for 3 hr, and mRNA levels were determined by QPCR.

(C–G) Colitis was induced with 5% DSS. Equal amounts of colonic nuclear extracts were analyzed by western blotting with the indicated antibodies (C). IL-6 in serum (D) and secreted from colon (E) was measured by ELISA. Expression of IL-6 (F) and S1PR1 (G) was determined in colons by QPCR.

Data are means ± SD. *p<0.05, **p<0.005.

See also Figure S2.