Fig. 1.

Three-step protocol of ESCs neuronal differentiation: a ESCs differentiation protocol outline; undiff undifferentiated. b ESCs aggregates at step I. c RT-PCR mRNA analysis of expanding ESCs (undifferentiated), or of ESCs at the end of step II, initially (step I) aggregated in two diverse conditions (SCM and CDMM), normalized on ESCs. d, e Oct4 (green) and Musashi-1 (red) immunocytodetection of ESCs at the end of step II, after CDMM (d) or SCM (e) aggregation in step I. f immunocytodetection for anti-acetylated-Tubulin antibody (Ntub, green) of ESCs aggregated and differentiated in CDMM, at the end of step III. Error bars standard error; p < 0.001 (Randomization test, REST software) for all SCM and CDMM values compared to ESCs values, except for Gata4 in CDMM, which was not significant