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. 2013 Feb 21;9(2):e1003281. doi: 10.1371/journal.pgen.1003281

Figure 8. Natural variations in the Ehd4 coding region among rice germplasm core collection.

Figure 8

(A) Haplotype network of the Ehd4 alleles in 86 rice accessions. Haplotype frequencies are proportional to the area of the circles. The proportion of wild rice and two cultivated subgroups (indica and japonica) in each haplotype is represented by different colors. (B) The polymorphic nucleotides of Hap_2 and Hap_3 of Ehd4 gene in the core collection. The number on the top shows the position of nucleotide polymorphisms in the coding region starting from the ATG start codon. (C) Geographic distribution of the cultivated rice accessions belonging to Hap_2 and Hap_3. (D) Flowering time of transgenic plants carrying two major haplotypes of Ehd4 driven by the maize Ubiquitin-1 promoter in ehd4 (Kita-ake background) and NIL carrying Ehd4 Hap3 compared with the 93-11 parental plants. T2 plants of two pUbi::Ehd4 Hap3 (#18 and #24) and two pUbi::Ehd4 Hap2 (#12 and #16) lines were measured (n = 15). All plants were grown in the natural long day field conditions. Values are means±s.d. (standard deviations) (n = 15). **Significant at 1% level; n.s., not significant.