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. 2013 Feb 21;8(2):e57547. doi: 10.1371/journal.pone.0057547

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© 2013 Persak and Pitzschke

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

Purified non-tagged MYB44 recombinant protein was incubated with MPK3-GST in the presence of y32P-ATP. Phosphorylation was detected by autoradiography. The known MAPK substrate, myelin basic protein (MBP) was used to document MPK3 functionality.