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. 2013 Feb 21;8(2):e57547. doi: 10.1371/journal.pone.0057547

Figure 10. Ectopic expression of MYB44 and S145 variants in stable transgenic Arabidopsis plants.

Figure 10

Protein extracts of 8-d-old seedlings were separated by SDS-PAGE. Transgene expression in MYB44 transgenic lines (2-3 independent lines shown per variant) was visualised by immunodetection, using rabbit anti-myc and IRDye800CW-coupled donkey-anti-rabbit (NEB Biolabs) as primary and secondary antibodies, respectively. Membranes were scanned with a LiCCOR Odyssee machine to detect bound antibody (800 nm channel, top) and to document equal sample loading (700 nm channel, bottom).