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. 2013 Feb 21;8(2):e57505. doi: 10.1371/journal.pone.0057505

Figure 9. ATP-induced bacterial killing activity was inhibited after aP2X7R ablation.

Figure 9

Plates displayed the survival L. anguillarum from macrophages treated with siRNA (A), aEPAb (B), or oATP (C). The histogram demonstrated the effects of siRNA, aEPAb, or oATP on bacterial killing. Macrophages were infected with live L. anguillarum after siRNA, aEPAb, or oATP treatment. L. anguillarum viability was examined through CFU assay after ATP treatment. Mouse IgG, scrambled siRNA, and PBS were added as controls for the respective treatments. (D) Reduction of L. anguillarum viability induced by ATP. After treatment with aEPAb or isotype IgG, cells were treated with ATP. Subsequently, cells were harvested at different time intervals, and bacterial viability was monitored through CFU assays. Data are representative of at least three independent experiments. *P<0.05; **P<0.01.