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. 2013 Feb 21;8(2):e57450. doi: 10.1371/journal.pone.0057450

Figure 1. Expression of neurotrophin receptor and ligands in primary cultures of cardiac fibroblasts and myocytes.

Figure 1

A) Primary cultures of cardiac fibroblasts express TrkA mRNA at levels higher than TrkC mRNA, comparable to the expression in cardiomyocytes. Cardiac fibroblasts and cardiomyocytes were isolated from adult female (left) or neonatal (right) C57BL/6 mice and their Trk transcripts quantified by qPCR. Data are combined from at least three separate experiments, each point in triplicate. B) Variable expression of neurotrophin mRNAs in primary cultures of adult or neonatal cardiac fibroblasts and myocytes. NGF, BDNF and NT-3 mRNAs were assessed by qPCR in primary cultures of adult female (left) or neonatal (right) cardiac fibroblasts (CF) and myocytes. Two technical replicates each on triplicate samples were normalized to HPRT and graphed as mean + s.d.