Figure 3. α-tomatine represses TNF-α-induced NF-κB dependent expression of pro-survival proteins.

PC-3 cells grown to 70–80 % confluence were treated with either 0.1% DMSO or 2 µM α-tomatine for 30 minutes, 10 ng/ml TNF-α was then added and the cells were incubated for a further 6 hours. Whole-cell extracts were prepared, and were analyzed by Western blot analysis using antibodies against Bcl-2, Bcl-xL, c-IAP1, c-IAP2, XIAP and survivin. The results shown here are representative of three independent experiments.