Figure 1. The principle of the 3-D worm tracker (3DWT).

(A) Experimental scheme of the stereoscopic recording. Two imaging assemblies at right angles with the same focal point are used to synchronously record images of C. elegans from two perpendicular directions. (B) Stereomatching of skeletons. The two X-Z and Y-Z skeletons, extracted from the two projection images of the worm, are combined using stereomatching to reconstruct the 3-D skeleton. (C) Volume rendering of the 3-D skeleton. The 3-D volume of the worm is rendered by assigning particles that are centered on the 3D skeleton points.