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. 2013 Feb 21;8(2):e57099. doi: 10.1371/journal.pone.0057099

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Limiting dilution tumorigenicity assay was performed with simultaneous injection of Matrigel. A. Control treated HCT-15 tumor line. B. SC-1 treated HCT-15 tumor line. Tumor weight for each mouse is depicted. Tumor formation was increased in SC-1 treated cells when 10 cells/mouse were injected (SC-1 treated: 5 of 5 tumors formed, Control treated: 2 of 5 tumors formed). At higher cell inocula, all mice formed tumors independent of treatment. Each line in both graphs represents growth of one tumor per mouse (A. A–E, B. F–J).