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. 2013 Feb 21;8(2):e57099. doi: 10.1371/journal.pone.0057099

Figure 2. Tumor Lines Treated with SC-1 Possess Characteristics Consistent with the CSC Phenotype.

Figure 2

The colon tumor lines were treated for five days with 0.1 µM SC-1, harvested and then analyzed for their ability to form colonies in soft agar, to alter expression of putative CSC surface markers, and to form spheres. A. The cloning efficiency of the colon tumor lines was determined following treatment with SC-1. In 5/7 tumor lines, SC-1 treated cells had statistically significant increases in cumulative colony forming unit (CFU) mass compared to control treated cells (***p<0.001, **p<0.1 *p<0.05, n = 3). In one instance (#p<0.05), SC-1 treatment reduced cloning efficiency. B. The CD133 positive subpopulation was increased 2.5-fold in SC-1 treated HT29 colon tumor line (p = 0.03, n = 3). The CD44+ CD24- subpopulation was increased after SC-1 treatment in the HCT-116 colon tumor line (*p<0.05, n = 3). The CD44+ subpopulation was increased significantly following SC-1 treatments (*p<0.05, n = 3) in SW-620 colon tumor line. C. Three tumor lines (COLO 205, HCT-116, HT-29) formed nonadherent spheres in the presence of 0.1 µM SC-1 cultured in standard media containing 5% FBS. These observations were also apparent at day 5 in culture. All images were prepared at 400× magnification.