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. 2013 Feb 21;8(2):e57099. doi: 10.1371/journal.pone.0057099

Figure 3. Effect of SC-1 on Protein Expression Levels of phospho-ERK1/2, and OCT4 in Colon Tumor Lines.

Figure 3

Colon tumor lines were treated with SC-1 (0.1 µM), harvested, lysed, and probed for the proteins of interest following electrophoresis in SDS-PAGE gels at the indicated time points. A. In the SC-1 treated HT29 tumor line, phospho-ERK1/2/total ERK1/2 protein levels were decreased to 67±0.06% of control value at 5 min, 56±0.06% of control value at 30 min, and 46±0.06% of control value at 1 hr (n = 3, p<0.05). B. Increased OCT4 protein expression due to SC-1 was dependent on the tumor line. Representative experiments are shown for Figure S4A–B (n = 2).