Table 4. Side-by-side comparison of H serotyping and MS-H typing of E. coli H types with the Orbitrap platform^a.

Strain Number	Previously recorded H-types with induced motility	Serotyping without induced motility	MS-H	Sequence coverage (%)
				Day 1	Day 2	Day 3
06-4319	H7	H7	H7	54	62	87
06-1139	H7	–	H7	87	84	87
07-1591	H7	–	H7	93	89	81
07-1756	H7	–	H7	85	87	87
EDL933	H7	H7	H7	90	71	88
90-2380	H7	–	H7	85	83	90
05-0958	H7	–	H7	81	62	88
09-0414	H7	–	H7	94	79	89
09-1349	H7	–	H7	94	88	90
09-1350	H7	–	H7	91	73	90
09-1351	H7	–	H7	77	72	88
09-1352	H7	–	H7	90	83	88
E169	H1	H1	H1	98	83	83
E170	H2	H2	H2	67	71	68
E171	H3	–	H3	92	90	86
E172	H4	H4	H4	89	99	88
E173	H5	–	H5	81	76	58
E174	H6	H6	H6	90	80	63
E176	H8	–	H8	90	90	77
E177	H9	H9	H9	80	77	80
E659	H10	–	H10	99	79	85
07-6285	H11	–	H11	85	84	80
E241	H12	–	–	–	–	–
E241Mb	H12	H12	H12	98	97	97
E752	H12	–	H12	98	91	97
E182	H14	H14	H14	47	81	55
E183	H15	H15	H15	73	81	79
E184	H16	–	H16	64	81	77
E185	H17	–	H17	74	85	84
E186	H18	H18	H18	58	68	65
09-0523	H19	H19	H19	66	89	88
E188	H20	H20	H20	81	73	81
E189	H21	H21	H21	95	98	95
E191	H23	H23	H23	65	67	79
E192	H24	H24	H24	72	65	74
E193	H25	H25	H25	66	73	68
E194	H26	H26	H26	98	75	85
E195	H27	H27	H27	71	63	69
E196	H28	–	H28	70	73	79
E197	H29	H29	H29	83	87	87
E198	H30	–	H30	78	78	79
E199	H31	–	H31	59	40	76
E200	H32	H32	H32	72	42	67
E201	H33	–	H33	70	65	75
E589	H34	H34	H34	65	66	67
E203	H35	–	H35	51	56	45
E204	H36	rough	H36	90	90	92
E205	H37	–	H37	96	91	85
E206	H38	H38	H38	97	98	86
E207	H39	H39	H39	83	68	61
E208	H40	H40	H40	97	92	91
E209	H41	–	H41	72	47	50
E210	H42	–	H42	48	–	36
E210Mb	H42	H42	H42	72	70	74
E211	H43	–	H43	90	89	89
E212	H44	H44	H44	78	74	76
E213	H45	–	H45	61	60	53
E214	H46	H46	H46	73	68	59
E346	H47	H47	H47	99	83	99
E247	H48	H48	H48	89	91	82
E248	H49	H49	H49	87	87	87
E372	H51	–	H51	84	63	56
E373	H52	–	H52	85	67	59
E374	H53	H53	H53	72	58	74
E377	H54	–	H54	81	68	70
E375	H55	–	H55	63	–	–
E375Mb	H55	rough	H55	71	71	65
E376	H56	–	H56	65	40	53
09-1760c	Untypeable	H21d	H21	97	95	96

-, serotyping titration or MS identification was not reached.

^aSerotyping and MS-H were performed concurrently from subcultures of single bacterial colonies. MS-H was repeated on two consecutive days; ^bmotility induction was performed for these inconsistent strains after initial MS-H; ^cuntypeable by serotyping although previous MS and PCR-based sequencing showed type H21; ^dusing designated antisera by MS-H.