Table 5. Diagnostic specificity, sample stability and run-to-run repeatability test results for MS-H with the Orbitrap platform^a.

Strain number	Previously recorded serotypes with motility induction	MS-H types without motility induction	Sequence coverage (%)
			Run 1	Run 2	Run 3
09-1336	NM	N/A	N/A	N/A	N/A
09-1337	NM	N/A	N/A	N/A	N/A
09-1338	NM	N/A	N/A	N/A	N/A
09-1339	NM	N/A	N/A	N/A	N/A
09-1345	NM	N/A	N/A	N/A	N/A
09-1346	NM	N/A	N/A	N/A	N/A
09-0411b	H11	H11	81	81	81
09-1342b	H11	H11	83	82	83
09-1344b	H11	H11	76	74	78
09-1765b	H11	H11	98	98	91
09-1767b	H11	H11	93	93	93
09-0409	H28	H28	70	79	75
09-0410	H28	H28	68	73	74
09-0416	H28	H28	76	77	78
09-0416	H28	H28	76	77	78
09-0412	H49	H49	88	89	89
09-0413	H16	H16	74	77	78
09-0415	H8	H8	98	91	90
09-1347	H19	H19	70	71	75
09-1348	H19	H19	67	69	68
09-1353	H25	H25	67	70	72
09-1354	H25	H25	64	74	78
09-1770	H19	H19	81	88	78
09-1774	H19	H19	88	88	88
09-1764	H19	H19	86	88	82
09-1766	H34	H34	59	59	59
09-1768	H14	H14	98	98	98
09-1769	H14	H14	98	98	98
09-1775	H25, then rough	H25	76	81	80

NM, non-motile; N/A, not attainable.

^aThe same flagella digest was tested by LC-MS/MS three times within a one-week period; ^ba two-year old residual digest was re-used.