| Diagnostic sensitivity |
Could reach 100% |
Could reach 100% |
| Diagnostic specificity |
Could reach 100% |
Could reach 100% |
| Analytical sensitivity |
Loop size of culture |
5 colonies |
| Analytical specificity |
Antigenic epitope dependent |
Ionization dependent |
| Read-out |
Agglutination titer observation by eyes; process may require several steps |
Protein and peptide sequences analyzed by software; one step identification |
| Motility induction |
Routinely required |
Not routinely required |
| Rapidity to get result |
3 to 5 days |
4 hr for a single sample |
| Ease of identifying rough strains |
Impossible |
Possible; motility induction can be used to obtain result |
| Result consistency |
Motility induction and antisera dependent |
Instrumentation and software dependent |
| Robustness and ruggedness |
Limited; largely performed manually; may require optimal antigen/antisera reaction conditions |
Good; largely performed by machine; can tolerate wide range of sample amounts and different instruments |
| Throughput |
Limited, largely performed manually; not easily repeated |
Good; LC-MS/MS can run day and night; easy to repeat and obtain a better result |
| Sample stability |
Variation in bacteria growth |
Protein digests are stable |
| Consumables and labor used |
Antisera, culture media,Craigie tubes; Half day of labor |
Trypsin, lysozyme, nano-LC columns; instrument service contract; MS routine runs, half hour of labor |
| System suitability/accessibility |
Reference labs/institutions with antiserum or antibody production |
Institutions or service labs with MS capability |
