Skip to main content
. 2013 Feb 21;8(2):e57391. doi: 10.1371/journal.pone.0057391

Figure 2. Klotho transfection reduced IC50 of both cell lines and inhibited Akt signal pathway.

Figure 2

A, B: Western blot assay showed the p-Akt expression was enhanced significantly in the cells resistant to cisplatin. C: MTT assay showed a great decrease in IC50 of klotho transfected cells. D: Western blot assay showed the elevated expression of klotho in cisplatin resistant cells. P-Akt expression was down-regulated in cisplatin resistant cells after klotho transfection. GAPDH served as an internal reference. Experiment was performed in triplicate and data were presented as mean±SD (*P<0.05)