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. 2013 Feb 21;8(2):e57391. doi: 10.1371/journal.pone.0057391

Figure 3. Klotho knockdown with specific shRNA increased IC50 and p-Akt expression in cisplatin resistant cells.

Figure 3

A: The ERCC1 and p-gp protein levels in cisplatin resistant cells were identified by western blot. B: A549DDP and H460DDP cells were transfected with klotho specific shRNA or scrambled RNA as a negative control. MTT assay showed an increased IC50 of cells transfected with shRNA. C: Western blot assay confirmed the klotho expression was down-regulated in the cells following transfection with shRNA. D: The p-Akt was also detected by western blot assay. Results showed the p-Akt expression was up-regulated significantly following shRNA tranfection. GAPDH served as an internal reference. Experiment was performed in triplicate and data wer presented as mean±SD (*P<0.05).