Figure 1. Generation of REX1^Ven/w human embryonic stem cells.

A) Schematic of the wild type human REX1 allele, targeting vector (REX1-VF2Pu TV) and targeted allele. B) Southern blot confirmation of targeting. C) Phase and fluorescence images of a REX1Ven/w hESCs. Scale bar = 100 microns. D) Flow cytometry on REX1^Ven/w cells grown for 7 days in undifferentiated hESC conditions with or without the addition of puromycin co-stained with TRA -1-60 or A2B5. Control inset. E & F) QRT-PCR analysis of pluripotency (E) and differentiation (F) gene transcript expression VEN+ populations isolated by FACS from undifferentiated REX1^Ven/w hESCs. All values are normalised relative to the VEN− population = 1.