Figure 2. Size-exclusion chromatography of SMOC-1 and S1EC in the presence and absence of glycosaminoglycans (GAGs).

(A) Elution profiles of S1FL under different conditions. The GAGs used were heparin (HP), heparin sulfate (HS), chondroitin sulfate (CS) and dermatan sulfate (DS). All runs were performed in 20 mM sodium phosphate buffer pH 7.4 containing 150 mM NaCl. (B) Elution profile of S1EC domain from a Superdex 200 SEC column in the presence and absence of heparin (HP). Both runs were performed in 20 mM sodium phosphate buffer pH 7.4 containing 150 mM NaCl. Elution volumes of standard proteins (Dalton Standards MS-II) are given in both diagrams.