Figure 3. Inhibition of NF-κB activation is associated with an increased endocrine/total graft area ratio in the islet graft.

A. The recipient graft-bearing kidneys from normoglycemic untreated mice, and Dox- or Bortezomib (BZB)-treated animals were removed 7 days after allogeneic transplantation, fixed in formaldehyde, thin-sliced and stained with hematoxylin/eosin solution. The border between the kidney and the graft is marked (Upper panel). Paraffin sections were stained for insulin (Lower panel). B. Using a fixed grid, the percentage of endocrine area was calculated from the total graft area. Results are the average of at least five non-consecutive sections incorporating the whole graft area. *p = 0.033 Dox vs control; **p = 0.0001 BZB vs control n = 3.