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. 2013 Feb 21;8(2):e56772. doi: 10.1371/journal.pone.0056772

Figure 5. LC-MS analysis of the hydroxylation activity of Qui15.

Figure 5

Before the hydroxylation reaction, all the samples were incubated with coexpression of Qui5 and holo-Qui18 for 2 hours to ensure the loading of L-tryptophan other than the negative control of (A). (A) L-tryptophan wasn’t incubated with coexpression of Qui5 and holo-Qui18 and hence all L-tryptophan was free instead of the loaded form; (B) L-tryptophan was incubated with coexpression of Qui5 and holo-Qui18 form but then no Qui15 was supplemented; (C) After the incubation of L-tryptophan and coexpression of Qui5 and holo-Qui18 for 2 hours, Qui15 was supplemented and an additional 60 min incubation was adopted; (D) the same as (C), except the additional incubation time was 120 min; (e) (2S,3S) β-hydroxytryptophan standard sample. The peak I is L-tryptophan and the peak II is β-hydroxytryptophan.