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. 2013 Feb 21;8(2):e56601. doi: 10.1371/journal.pone.0056601

Figure 2. Myotube Akt phosphorylation, human primary macrophage cholesterol content and THP-1 macrophage JNK phosphorylation. (a).

Figure 2

Phosphorylation of Akt at Serine-473 in human primary skeletal myotubes treated as for Figure 1, n = 6/group. (b) Total intracellular cholesterol (free cholesterol and cholesteryl esters) in human primary macrophages after treatment with vehicle (PBS) or 75 µg/ml acLDL/10 µg/ml Sandoz with or without 50 µg/ml HDL for 18 hours, n = 6/group. (c) Phosphorylation of JNK in THP-1 macrophages treated with vehicle or 75 µg/ml acLDL/10 µg/ml Sandoz with or without 50 µg/ml HDL for 1, 2 and 4 hours. n = 9/group; data are presented as mean ± SEM; *indicates significantly different from Con at the corresponding time point, P<0.05.