Table 2. Concentration (pg/ml, mean ± SEM) of cytokines measured in media taken from primary human macrophages after incubation with vehicle (PBS) or acLDL with or without HDL.
Cytokine (pg/ml) | Vehicle | acLDL | acLDL+HDL |
GM-CSF | ND | 483±243 | 520±241 |
IL-8 | 2191±1077 | 62931±22647* | 71201±31284* |
TNF-α | 27±10 | 3847±1055* | 2784±994* |
IL-1 RA | 71536±29032 | 122636±36818 | 120776±41493 |
MCP1 | 3126±1319 | 7725±2989 | 8837±3736 |
MIP-1α | 99±27 | 56225±16782* | 44473±13117* |
MIP-1β | 88±47 | 17101±5508* | 18183±6645* |
RANTES | 28±9 | 1042±303* | 909±244* |
IL-10 | ND | 684±144 | 589±130 |
Media was added to skeletal myotube cultures at a concentration of 2% (diluted in regular media). *indicates significantly increased above Con, P<0.05. ND: Not detectable.
GM-CSF, granulocyte-monocyte colony-stimulating factor; IL-8, interleukin-8; TNF-α, tumor necrosis factor-α; IL-1ra, interleukin-1 receptor antagonist; MCP1, monocyte chemoattractant protein 1; MIP-1α, macrophage inflammatory protein-1α; MIP-1β, macrophage inflammatory protein-1β; RANTES, regulated upon activation normal T-cell expressed and secreted; IL-10, interleukin-10.