Figure 5. Tanshinone IIA slowed down the degradation of p27 protein in PASMCs under hypoxic condition.

(A) Representative western blot for p27 protein in PASMCs at different time point after cycloheximide (50 µg/mL) added under hypoxia (3%) or hypoxia plus tanshinone IIA (10 µg/mL) condition. Beta-actin was used as control. Summarized data from three experments were shown panel (B). Data were normalized by dividing the protein level at time zero. P27 protein degraded fast but tanshinone IIA significantly slowed down the degradation of p27 protein (*P<0.05 vs corresponding time point in the same group. mean ± SEM, n = 3). CHX represents cycloheximide (50 µg/mL).