Figure 3.
Effect of CRH in dexamethasone-treated NS/PCs proliferation and apoptosis. (a) Representative figures of 5-bromo-2-deoxyuridine (BrdU)-labeled cells (red) counted 24 h after treatment with or without CRH after pretreatment with dexamethasone. Dexamethasone was added in cell culture 1 h before CRH. 4′6-diamidino-2-phenylindole (DAPI) staining was applied for visualization of total cell abundance. Scale bar=200 μm. (b) Graph that depicts total number of the BrdU-positive cells count in 180 × 180 μm2 areas. Data are shown as mean±s.e.m. (n=4). *P<0.01, **P<0.01 versus non-CRH-treated cells. (c) Effect of CRH on dexamethasone-treated NS/PCs apoptosis induced by serum deprivation for 24 h.Representative images of terminal transferase dUTP nick-end labeling (TUNEL)-stained NS/PCs (red) combined with DAPI nuclear staining (blue) after treatment with or without CRH and/or dexamethasone. Scale bar=200 μm. (d) Graph depicts quantification of the TUNEL-positive cells count in 180 × 180 μm2 areas. Data represent the mean±s.e.m. (n=4). *P<0.05, **P<0.01.