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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Photochem Photobiol. 2012 Dec 10;89(2):326–331. doi: 10.1111/php.12009

Figure 3.

Figure 3

(a) pKa titration curve expressed as a fluorescence intensity ratio of 770 nm to 820 nm at different pH values. Calculated pKa = 5.1 ± 1.3. (b) Fluorescence spectra of dye 3 bound to albumin in acidic and neutral media. Fluorescence intensity show sharp increase at 790 nm in acidic media.