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. Author manuscript; available in PMC: 2014 Feb 1.

Published in final edited form as: Mol Cancer Res. 2012 Dec 5;11(2):173–181. doi: 10.1158/1541-7786.MCR-12-0065

CBP depletion shortens the DNA repair time in neuroblastoma cells. (A) SH-SY5Y cells transfected with CBP siRNA or control siRNA were irradiated at 2Gy. At different times following IR, immunofluorescence for γH2AX was conducted as described in the Method section. Zero, 3, 5 and 7 hour after IR are shown in (A). (B) Compilation of the number of foci ofγH2AX per cells after IR described in (A) at times indicated. The results are expressed as Mean±SD (N=3, 100 foci per sample). (C) and (D) SH-SY5Y cells were transfected with CBP siRNA or control siRNA. Two days after transfection, the cells were irradiated at 10Gy. At 0, 1, and 3 hours after IR the cells were subjected to the Comet assay as described. The fluorescent microscopy staining with SYBR green was shown in (C). The average comet tail moment (percentage of DNA in tail length) was scored in three different fields (at least 50 comets per field) shown in (D). The results are expressed as Mean±SD (n=3).

CBP depletion shortens the DNA repair time in neuroblastoma cells. (A) SH-SY5Y cells transfected with CBP siRNA or control siRNA were irradiated at 2Gy. At different times following IR, immunofluorescence for γH2AX was conducted as described in the Method section. Zero, 3, 5 and 7 hour after IR are shown in (A). (B) Compilation of the number of foci ofγH2AX per cells after IR described in (A) at times indicated. The results are expressed as Mean±SD (N=3, 100 foci per sample). (C) and (D) SH-SY5Y cells were transfected with CBP siRNA or control siRNA. Two days after transfection, the cells were irradiated at 10Gy. At 0, 1, and 3 hours after IR the cells were subjected to the Comet assay as described. The fluorescent microscopy staining with SYBR green was shown in (C). The average comet tail moment (percentage of DNA in tail length) was scored in three different fields (at least 50 comets per field) shown in (D). The results are expressed as Mean±SD (n=3).