Figure 5.

The expression of STAT signaling pathway components was altered by REA knockdown during hESC differentiation. Expression of the STAT signaling pathway components, IL-11, STAT3, GP130, and PRLR2, was enhanced by knockdown of REA using siRNA during hESC decidualization (A), whereas the mRNA levels of several suppressors of cytokine signaling (SOCS1, SOCS3, and SOCS5) were reduced, as shown by q-PCR analysis (B). Elevated total and phosphorylated STAT3 protein, as well as reduced SOCS3 protein, in hESC after REA knockdown was observed by immunofluoresence in hESC at 96 hours of differentiation (C). Immunofluorescence of total STAT3, phospho-STAT3 (p-STAT3), or SOCS3 was merged with DAPI staining and presented under a higher magnification (×100) (C, right) to show cellular location of these proteins. Scale bar, 100 μm. *P < .05.