Figure 4.

Mn supplementation improves glucose tolerance and insulin secretion in a high-fat diet-induced diabetic model. High-fat or normal chow diets were given to age-matched C57BL/6J (3–4 months old) male mice for a month. Maintaining the same diet, saline or Mn was then administered ip for 8 weeks. A, Tail glucose levels were determined at the indicated times after the glucose challenge. B, Area under the glucose curve (AUC) of the glucose tolerance test was calculated. C, Body weights were determined of the mice on normal chow or the high-fat diet, saline or Mn treated. D, HOMA-IR. E, Serum insulin levels were determined before and 30 minutes after a glucose challenge. F, HOMA-B. A, C, and E were analyzed by the bivariate ANOVA (2 way ANOVA). Different letters show statistical differences (P < .05). B, D, and F were analyzed by the Student t test (2 tail), comparing the difference between saline-IP vs MN-IP groups. ***P < .001, *P < .05, 8–11 mice/group (n = 3–11 mice/group for insulin measurement). Values are represented as the mean ± SEM.