Figure 1.

E2 regulates whole-body energy expenditure. (A) Whole-body O₂ consumption. (B) Whole-body CO2 production. (C) Whole-body energy expenditure. (D) mRNA UCP-2 expression in liver. (E) O₂ consumption in isolated hepatocytes. (F) mRNA UCP-1, Cidea, and PRDM16 expression in WAT. Significance between groups was determined by 1-way ANOVA (n = 8). Data represent the mean ± SEM. *P < .01 compared with SHAM, #P < .01 compared with OVX. AU, arbitrary units; OCR, oxygen consumption rate; VO₂;VCO₂, volume of O₂, volume of CO₂.