Fig. 1.

Expression of and activation of endogenous TRPM7 channels in HEK293, RAW264.7, and BMMs. (A) Whole cells lysates were collected from cells stimulated with RANKL for indicated time. TRPM7 was blotted with its antibody. (B) mRNA expression of TRPM7 were decreased after transfection of siTRPM7 in HEK293 and RAW264.7 cells. (C) Activation of endogenous TRPM7-mediated currents by a voltage ramp (-100 mV to +100 mV in 50-ms intervals, V_h=0 mV), used to determine current-voltage relations in cells. TRPM7 currents were activated by 0 mM [Mg²⁺]_e and these effects diminished in TRPM7 knock-down cells. (D) The amplitude of endogenous TRPM7-mediated currents at -80 and +80 mV in HEK293 and RAW264.7 cells. Data were expressed as the mean±SEM. ^**p<0.01, ^*p<0.05 compared with control.