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. 2013 Jan 25;32(4):524–537. doi: 10.1038/emboj.2013.1

Figure 3.

Figure 3

Goliath- or Godzilla-induced endosomal enlargement is dependent on their ubiquitin ligase activity. (A, B) HEK293 cells were transfected with Goliath-C-GFP, ligase-dead Goliath-C-GFP (A), Godzilla-C-GFP, or ligase-dead Godzilla-C-GFP (B), and stained for EEA1 and ubiquitylated proteins (FK2). Strong ubiquitylation was observed on enlarged endosomes upon expression of ligase-active forms of Goliath and Godzilla, but not ligase-dead forms. Lower panels show an expanded view of dotted boxed area of upper image. Bar 10 μm. (C) Quantification of induction of enlarged endosomes by Goliath and Godzilla. The number of transfection-positive (GFP-positive) HEK293 cells containing giant endosomes was quantified. Results are given as means±s.d. of four independent experiments.