Figure 4.

Loss of Godzilla function leads to a robust accumulation of Rab5 endosomes. (A) RNAi-mediated knockdown of Godzilla driven by MS1096-Gal4 induces the formation of Rab5-positive giant endosomes in the Drosophila wing disc. Control is MS1096-Gal4-driven UAS-GFP^CAAX. Rab5 (red) and GFP (green) are shown. Left panels are lower magnification overview of wing discs. Right panels are close-up view of the centre of wing pouch. Enlarged Rab5-positive endosomes are observed with godzilla RNAi but not in control. (B) Godzilla mutant animals exhibit enlarged Rab5-positive endosomes. Wing discs of late third instar larvae were visualized with antibodies towards Rab5 (red) as well as phalloidin to visualize F-actin (green). Dotted box shows an expanded view of each panel. Rab5-positive endosomes are enlarged and accumulated in godzilla mutant wing discs, but not in control (w¹¹¹⁸). Bar 10 μm. (C) Rab5 endosomes accumulate to high levels in godzilla mutant wing discs. Both images were taken at identical exposure times. The results shown here are typical images from at least four independent experiments.