Figure 7.

Kif3a is required for ciliary basal foot formation. Serial ultrathin sections electron micrographs of basal bodies in (A) WT, (B) Kif3a^−/− and (C) Ift88^−/− MEFs. Two consecutive sections reveal the presence of a primary cilium in three WT cells. Primary cilia are absent in Kif3a and Ift88 mutant MEFs. Ciliary basal feet (asterisks) are detected in the subdistal region of basal bodies in WT and Ift88^−/− MEFs, but are not found in Kif3a^−/− MEFs. Basal feet in at least 10 serially sectioned samples were analysed per experiment (n=3). Scale bars indicate 200 nm for all images.